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There are chemical that can be put into the sewer pipe to kill roots. The only way you will know now bad it is will be to have it scoped. May just be a section where roots entered and the root growing inside the pipe from there.
If it is only the upstairs bathroom, and all the other drains work fine, then the problem is in the upstairs plumbing, not in the sewer line in the yard.
Did you run the snake thru the clean out in the yard? Getting roots, I'm guessing you did. Did you by chance also snake down the vent pipe on the roof?
90' from a yard clean out is a long ways, but depending on how far your house is from the street, it may not have even reached the city sewer.
I would call a plumber.
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