U.S. CitiesCity-Data Forum Index
Go Back   City-Data Forum > General Forums > Science and Technology
Please register to participate in our discussions with 2 million other members - it's free and quick! Some forums can only be seen by registered members. After you create your account, you'll be able to customize options and access all our 15,000 new posts/day with fewer ads.
View detailed profile (Advanced) or search
site with Google Custom Search

Search Forums  (Advanced)
Old Today, 11:20 AM
Location: Macon, Georgia
500 posts, read 208,979 times
Reputation: 304


Reply With Quote Quick reply to this message

Old Today, 11:57 AM
38,023 posts, read 39,396,447 times
Reputation: 15358
This will cause chaos in sports competition. Mark Phelps has a condition where his body produces half the lactic acid than other swimmers of his stature. Lactic acid builds in muscles during exertion and causing soreness and weakness, it's the bodies defense against overexertion. This gives Phelps a huge advantage over other swimmers and there is no way for other swimmers to compensate for it. Just to be clear I'm suggesting his achievements need an asterisk.

The issue is in the future how does one tell if this or something else that gives an athlete an advantage wasn't edited in or naturally occurring?
Reply With Quote Quick reply to this message
Old Today, 07:18 PM
Location: Pacific 🌉 N, 🌄W
10,630 posts, read 4,471,160 times
Reputation: 6778
Gene editing is not as advanced as these stories tend to portray.

AP Exclusive: US scientists try 1st gene editing in the body

First US attempt to cure a rare disease with genome editing fails miserably
Reply With Quote Quick reply to this message
Old Today, 08:36 PM
4,521 posts, read 2,938,164 times
Reputation: 5435
Originally Posted by Matadora View Post
Gene editing is not as advanced as these stories tend to portray.
I work in research (make viruses) and we have an entire CRISPR library for the human genome. I think it cost us $100k with shipping and tax. In theory it sounds great, just cut out the "defective" gene, but there's so much unknown on what effect it will/could have on the rest of the person/animal/cell.

We sell individual vectors to researchers doing all sorts of stuff, mostly cancer related and they are just doing in vitro (within cells) stuff and it works great. Taking that next step is the tricky part. Most researchers we deal with use it as a "knockout" and are going gene by gene to see what happens to the cancer cell by turning off a specific gene, it's very laborious and time consuming, but the CRISPR does allow them to speed up the process. In the past, it could take them 6 months to create a knockout, now they just order whatever vector they need for that specific gene and I can have it to them in 30 hours and away they go.

So the company we bought our library from, basically took the entire human genome and divided it up into segments representing every gene. The library is actually made up of gRNA (guide RNA) which corresponds to a specific DNA sequence in the genome. They estimate the human has maybe 20,000 genes and the way the library is created, it's not perfect, so there is overlap and they tried to create at least 3 vectors per gene. So that's why the library consists of, I think, 650 or so 96-well plates (62,400 wells total), with each vector (representing a certain gene or part of gene) frozen in an E. coli stock in every well.

The so-called "magic" of the system is the Cas9 protein, which takes the gRNA in each vector and will find that location it matches on the DNA by unzipping it and will make a cut on one end and the other end and remove that segment. The researchers that discovered this actually found it as a defense mechanism used by bacteria against bacteriophages (viruses that only infect bacteria or archea).

There's some amazing videos on YouTube that explain the CRISPR process and even dumb it down for the non-scientific. There was a huge patent lawsuit of UC Berkeley vs. the Broad Institute of the Massachusetts Institute of Technology over who "discovered" CRISPR first.

Broad Institute Fends off CRISPR Patent Lawsuit, at Least for Now


Last edited by cjseliga; Today at 10:06 PM..
Reply With Quote Quick reply to this message
Please register to post and access all features of our very popular forum. It is free and quick. Over $68,000 in prizes has already been given out to active posters on our forum. Additional giveaways are planned.

Detailed information about all U.S. cities, counties, and zip codes on our site: City-data.com.


Quick Reply

Over $104,000 in prizes was already given out to active posters on our forum and additional giveaways are planned!

Go Back   City-Data Forum > General Forums > Science and Technology
Follow City-Data.com founder on our Forum or

All times are GMT -6.

2005-2019, Advameg, Inc.

City-Data.com - Archive 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 - Top