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Old Today, 05:29 AM
 
Location: NJ
16,545 posts, read 25,122,374 times
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The link I used doesn't work, thankfully I copied the directions. I found a you tube video that shows the process



https://www.youtube.com/watch?v=EvD9pZ2wH4Q


Quote:
Originally Posted by Roselvr View Post
Using Q-tips to do ancestry DNA for people that can't spit in the vial or kids

How to do an AncestryDNA test WITHOUT spit

SUPPLIES:

Distilled water
3 clean disposable cups
1 measuring syringe
Simply Saline Adult Nasal Mist (DO NOT get saline with other additives or medications)
GUM Go-Betweens ProxaBrush Cleaners
Your DNA spit kit of choice (I used AncestryDNA)

1: Create Your Artificial Saliva Solution

Pour a fair amount of distilled water into one of the clean cups.
Spray a small amount of saline into another clean cup until it pools in the bottom.
Measure 99ml of distilled water into the third clean cup.
Measure 1ml of saline into the third clean cup.
Discard the two unmixed cups.
2: Take Your Sample

Now the fun part! You will need to obtain your sample.

Measure approximately 5ml of your artificial saliva and fill the test tube 2-3mm ABOVE the fill line. As you remove swabs, liquid will be removed from the container, so overfilling it very slightly will not be a problem.
Swab the GUM brushes on the cheeks 10-20 seconds, then place tip down into the DNA tube to soak for another 10-20 seconds. You may “stir” them in the tube as well. Remove the swab carefully allowing the liquid to drip off of it and into the vial, and scraping the swab along the edge to ensure as much is left behind as possible.
Repeat above until all 8 swabs have been used. While swabbing, it is best to leave the previous swab in the solution to soak.
3: Proceed as normal

At this point, your saliva sample should look identical to actual saliva. It’s actually pretty cool. If you tilt and swish it near a light source, you should be able to see debris from the cheeks and it should be thick and slimy just like actual spit. Screw on the cap with the stabilizing liquid, place it in the pouch, seal the box and ship it in!
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