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Old 12-31-2015, 11:51 PM
 
Location: Singapore
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I have a question on cloning; this is very much a biological life-sciences question; so i don't know if anyone would be able to answer this question.

Cloning works by taking the the DNA from the cell of an adult animal (i.e the original DNA sample), called the "donor," is inserted into an egg cell from another animal of the same species.

My question is if this is done repeatedly, would there be damage to the original DNA sample over time?
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Old 01-01-2016, 07:24 AM
 
Location: Lakewood NJ/Murrells Inlet SC/ N. Naples FL/Swainton NJ
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There ends up being no "original DNA" sample, as the DNA is replicated as the cells divide and multiply. Of course damage can happen from radiation or chemical agents, but that is a rare occurrence, especially in a womb environment.
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Old 01-01-2016, 12:02 PM
 
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Will cloned animals suffer premature aging

For those less scientifically inclined:

Myths about Cloning
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Old 01-08-2016, 01:45 AM
 
Location: Pacific 🌉 °N, 🌄°W
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Quote:
Originally Posted by tigerbalm1985 View Post
My question is if this is done repeatedly, would there be damage to the original DNA sample over time?
Not exactly sure what you are asking. Can you please explain what you mean by this..."if this is done repeatedly."

The DNA used in cloning is a one time transfer at the time of cloning.

This site explains the principals of cloning.

What is Cloning
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Old 01-08-2016, 02:57 PM
 
Location: Pacific 🌉 °N, 🌄°W
11,757 posts, read 5,756,082 times
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Quote:
Originally Posted by tigerbalm1985 View Post
I have a question on cloning; this is very much a biological life-sciences question; so i don't know if anyone would be able to answer this question.

Cloning works by taking the the DNA from the cell of an adult animal (i.e the original DNA sample), called the "donor," is inserted into an egg cell from another animal of the same species.

My question is if this is done repeatedly, would there be damage to the original DNA sample over time?
I think you have might have cloning a bit confused.

There are several types of cloning and they all use different techniques.

If you are asking about Reproductive Cloning it's not the transfer of an isolated DNA molecule or Recombinant molecule...its the transfer of the entire Nucleus (where the DNA resides).

Reproductive cloning is the production of a genetic duplicate of an existing organism. A human clone would be a genetic copy of an existing person.

Somatic cell nuclear transfer (SCNT) is the most common cloning technique. SCNT involves putting the nucleus of a body cell into an egg from which the nucleus has been removed. This produces a clonal embryo, which is triggered to begin developing with chemicals or electricity. Placing this cloned embryo into the uterus of a female animal and bringing it to term creates a clone, with genes identical to those of the animal from which the original body cell was taken.


If you're asking about Plasmid Cloning by Restriction Enzyme Digest then the process is completely different.
  1. Isolate DNA from an organism.
  2. Cut the DNA into pieces using a restriction enzyme, which is an enzyme that recognizes and cuts within a specific DNA a sequence - and insert (ligate) each piece, individually, into a cloning vector cut with the same restriction enzyme to make a recombinant DNA molecule, i.e. a DNA molecule constructed in vitro containing sequences from two or more distinct DNA molecules.
  3. Introduce (transform) the recombinant DNA molecules into a host (vector) such as E. coli. Replication of the recombinant DNA molecule - which is the process of molecular cloning - occurs in the host cell, producing many identical copies called clones.

*Throughout any cloning process controls are done to ensure quality and purity.* You would be wasting your time cloning a mistake if you don't perform quality checks all throughout the process.

This gives site gives a good overview of this type of cloning. Plasmid Cloning by Restriction Enzyme Digest

I hope this helps

Last edited by Matadora; 01-08-2016 at 03:06 PM..
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